Small nucleolar RNA sR8

In molecular biology, Small nucleolar RNA sR8 is a non-coding RNA belonging to the C/D box class of snoRNAs. sR8, along with other C/D box snoRNAs, performs 2′-O-methylation of ribose on a target strand of ribosomal RNA.[1][2] Targeting is achieved through the C and D box components, which are short sections of conserved sequences, as well as C'/D' boxes (internal equivalents of the C and D boxes). These sequences base-pair with nucleotides in the target rRNA to direct their methylation.[3]

sR8
sR8 consensus sequence
Identifiers
SymbolsR8
RfamRF01311
Other data
RNA typesnoRNA; CD-box
Domain(s)Sulfolobaceae, Thermococcaceae
PDB structuresPDBe

sR8 RNA operates alongside protein components in a ribonucleoprotein (RNP) complex. sR8 is able to guide the methylation of targets found within structured stem-loops, which implies that a component of the complex is able to unwind such structures.[3]

As a model system

sR8 is considered a 'model'[3] C/D box sRNA and has been used in several experiments investigating the nature of C/D box snoRNAs and ribonucleoprotein complexes.[4][5] It is particularly useful in studies because the RNP can be entirely constructed in vitro.[3]

sR8 RNA from the hyperthermophile Methanocaldococcus jannaschii has been used to elucide the 3-dimensional structure of a C/D box RNP.[6] The structure was unexpectedly found to be dimeric, wherein two RNA molecules bind with several proteins in the same complex.[6][7]

References
  1. Galardi, S.; Fatica, A.; Bachi, A.; Scaloni, A.; Presutti, C.; Bozzoni, I. (October 2002). "Purified Box C/D snoRNPs Are Able to Reproduce Site-Specific 2'-O-Methylation of Target RNA in Vitro". Molecular and Cellular Biology. 22 (19): 6663–6668. doi:10.1128/MCB.22.19.6663-6668.2002. PMC 134041. PMID 12215523.
  2. Kiss-Laszlo, Z; Henry Y; Bachellerie JP; Caizergues-Ferrer M; Kiss T (1996). "Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs". Cell. 85 (7): 1077–1088. doi:10.1016/S0092-8674(00)81308-2. PMID 8674114. S2CID 10418885.
  3. Appel CD, Maxwell ES (June 2007). "Structural features of the guide:target RNA duplex required for archaeal box C/D sRNA-guided nucleotide 2'-O-methylation". RNA. 13 (6): 899–911. doi:10.1261/rna.517307. PMC 1869030. PMID 17438123.
  4. Tran, E.; Zhang, X.; Lackey, L.; Maxwell, E. S. (2005). "Conserved spacing between the box C/D and C'/D' RNPs of the archaeal box C/D sRNP complex is required for efficient 2'-O-methylation of target RNAs". RNA. 11 (3): 285–293. doi:10.1261/rna.7223405. PMC 1370718. PMID 15661846.
  5. Rashid R, Aittaleb M, Chen Q, Spiegel K, Demeler B, Li H (October 2003). "Functional requirement for symmetric assembly of archaeal box C/D small ribonucleoprotein particles". J. Mol. Biol. 333 (2): 295–306. doi:10.1016/j.jmb.2003.08.012. PMID 14529617.
  6. Bleichert F, Gagnon KT, Brown BA, et al. (September 2009). "A dimeric structure for archaeal box C/D small ribonucleoproteins". Science. 325 (5946): 1384–7. Bibcode:2009Sci...325.1384B. doi:10.1126/science.1176099. PMC 2975540. PMID 19745151.
  7. "Study Reveals Unexpected Ancient Cellular Structure". PhysOrg.com.
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