BAF agar

BAF agar or biotin-aneurin-folic acid agar is a type of agar growth medium containing peptones. It is used to grow cultures of mycorrhizal fungi.[1] It was first described by A.J.P. Oort in Nutritional requirements of Lactarius species and cultural characters in relation to taxonomy in 1981.[1] The acidic pH (5.8-6.3) of BAF agar inhibits bacterial growth.[2]

Typical composition

BAF agar typically contains:[2]

30.0 g/L glucose
2.0 g/L peptone
0.2 g/L yeast extract
0.5 g/L KH₂PO₄
0.5 g/L MgSO₄.7 H₂O
10.0 mg/L FeCl₃.6 H₂O
1.0 mg/L ZnSO₄.7 H₂O
5.0 mg/L MnSO₄
100.0 mg/L CaCl₂.2 H₂O
50.0 μg/L thiamine HCl
1.0 μg/L biotin
100.0 μg/L folic acid
50.0 μg/L inositol
15 g/L agar

References

Águeda, Beatriz; Parladé, Javier; Fernández-Toirán, Luz Marina; Cisneros, Óscar; de Miguel, Ana María; Modrego, María Pilar; Martínez-Peña, Fernando; Pera, Joan (October 2008). "Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)". Mycorrhiza. 18 (8): 443–449. doi:10.1007/s00572-008-0192-3. ISSN 0940-6360. PMID 18695982. S2CID 11233267.
"CBS List of Media" (PDF).

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[:1-1] Águeda, Beatriz; Parladé, Javier; Fernández-Toirán, Luz Marina; Cisneros, Óscar; de Miguel, Ana María; Modrego, María Pilar; Martínez-Peña, Fernando; Pera, Joan (October 2008). "Mycorrhizal synthesis between Boletus edulis species complex and rockroses (Cistus sp.)". Mycorrhiza. 18 (8): 443–449. doi:10.1007/s00572-008-0192-3. ISSN 0940-6360. PMID 18695982. S2CID 11233267.

[:0-2] "CBS List of Media" (PDF).