Urine organic acids

Urine organic acids is a medical diagnostic test that measures organic acid metabolites in the urine. The metabolites can come from host cells or from flora. The test can be used to exclude the possibility that a person has an inborn error of metabolism, usually one of the organic acidemias. It is also used to look for problems with nutrition or evidence of certain infections or bacterial overgrowth. The usual method of analysis is tandem mass spectrometry.[1]

Urine organic acids
Purposemeasures organic acid metabolites in urine


Urine organic acid analysis profile: The 3 prominent peaks on the left are from left to right: B-hydroxybutyrate, Acetoacetate (with TMS derivative 1), Acetoacetate 2nd peak (with TMS derivative 2). TMS = trimethylsilyl derivative reagent, PDA = pentadecanoic acid (internal standard).
GC-MS total ion chromatogram of a urine sample in a patient with diabetic ketoacidosis

References
  1. Jones, P. M.; Bennett, M. J. (2010). "Urine Organic Acid Analysis for Inherited Metabolic Disease Using Gas Chromatography-Mass Spectrometry". Clinical Applications of Mass Spectrometry. Methods in Molecular Biology. Vol. 603. pp. 423–431. doi:10.1007/978-1-60761-459-3_41. ISBN 978-1-60761-458-6. PMID 20077094.


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