Terpentetriene synthase

Terpentetriene synthase (EC 4.2.3.36, Cyc2) is an enzyme with systematic name terpentedienyl-diphosphate diphosphate-lyase (terpentetriene-forming).[1][2][3] This enzyme catalyses the following chemical reaction

terpentedienyl diphosphate

\rightleftharpoons

terpentetriene + diphosphate

This enzyme requires Mg²⁺ for maximal activity but can use Mn²⁺, Fe²⁺ or Co²⁺ to a lesser extent.

References

Dairi T, Hamano Y, Kuzuyama T, Itoh N, Furihata K, Seto H (October 2001). "Eubacterial diterpene cyclase genes essential for production of the isoprenoid antibiotic terpentecin". Journal of Bacteriology. 183 (20): 6085–94. doi:10.1128/jb.183.20.6085-6094.2001. PMC 99688. PMID 11567009.
Hamano Y, Kuzuyama T, Itoh N, Furihata K, Seto H, Dairi T (October 2002). "Functional analysis of eubacterial diterpene cyclases responsible for biosynthesis of a diterpene antibiotic, terpentecin". The Journal of Biological Chemistry. 277 (40): 37098–104. doi:10.1074/jbc.m206382200. PMID 12138123.
Eguchi T, Dekishima Y, Hamano Y, Dairi T, Seto H, Kakinuma K (July 2003). "A new approach for the investigation of isoprenoid biosynthesis featuring pathway switching, deuterium hyperlabeling, and 1H NMR spectroscopy. The reaction mechanism of a novel streptomyces diterpene cyclase". The Journal of Organic Chemistry. 68 (14): 5433–8. doi:10.1021/jo026728a. PMID 12839434.

Terpentetriene+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)

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Carbon–oxygen lyases (EC 4.2) (primarily dehydratases)
4.2.1: Hydro-Lyases	Carbonic anhydrase Fumarase Aconitase Enolase Alpha Enolase 2 Enoyl-CoA hydratase/3-Hydroxyacyl ACP dehydrase Methylglutaconyl-CoA hydratase Tryptophan synthase Cystathionine beta synthase Porphobilinogen synthase 3-Isopropylmalate dehydratase Urocanase Uroporphyrinogen III synthase Nitrile hydratase
4.2.2: Acting on polysaccharides	Hyaluronate lyase
4.2.3: Acting on phosphates	Threonine synthase
4.2.99: Other	Carboxymethyloxysuccinate lyase Hydroperoxide lyase

Enzymes
Activity	Active site Binding site Catalytic triad Oxyanion hole Enzyme promiscuity Diffusion-limited enzyme Coenzyme Cofactor Enzyme catalysis
Regulation	Allosteric regulation Cooperativity Enzyme inhibitor Enzyme activator
Classification	EC number Enzyme superfamily Enzyme family List of enzymes
Kinetics	Enzyme kinetics Eadie–Hofstee diagram Hanes–Woolf plot Lineweaver–Burk plot Michaelis–Menten kinetics
Types	EC1 Oxidoreductases (list) EC2 Transferases (list) EC3 Hydrolases (list) EC4 Lyases (list) EC5 Isomerases (list) EC6 Ligases (list) EC7 Translocases (list)