Skin Horse

Dr. Dennis "Tip" Wilkin, a relatively new field operative and the only fully human employee of Project Skin Horse, was a decorated former captain of the U.S. Army, a trained psychologist, a passionate cross-dresser and an almost supernatural seducer. Tip was essentially a research psychologist, but in an emergency he could wield therapy puppets.

Captain's Fancy Valentine Sweetheart was a genetically modified sapient dog, created by a mad scientist. She came to work for Project Skin Horse as a field operative. She was generally the most clear-thinking and rational team member, but periodically exhibited feelings of insecurity.

Unity was a "necrotic" being: dead tissue animated through a secret military process. A field operative for Project Skin Horse, she was Sweetheart's best friend. Unity was developed to be a bio-weapon, but proved to be too hard to control and was therefore moved to Project Skin Horse. Unity was good-natured, liked guns, and was inclined to use gratuitously unnecessary violence. Her safety phrase, "Blueberry waffles", to suspend her aggression patterns, was only revealed by Anasigma after Project Skin Horse had spent a fortune on obedience classes.

Unity as a creature was actually a nanotechnology ooze that controlled the body it inhabited, as seen in the "Once and Future" story arc. As such, she could be transferred (with help) into another body or creature, although living bodies would reject her after a time, usually by vomiting her out. Her “normal” body was an amalgam of various human corpses of varying ethnicities, and several times throughout the series, she was seen to add, remove, or adapt to new body parts.

Unity was also seen to gain intelligence proportional to the amount of brains she consumed, although social graces were not included with this increase. During the story arc "Railway Children," she consumeed a vast quantity of artificially produced brains, and with it gained superhuman intelligence levels. She repeated this during the events of "My brother Sam is Dead," only to use her insight to make comments about her teammates' behavior that left them distrusting of her in this heightened state.

The administrative leader of Project Skin Horse, Gavotte was a collective being consisting of a swarm of bees that thought with a single female mind. Most of the other agency employees were nervous around her—Tip in particular since he was fatally allergic to bee stings.[5]

A 19th century wind-up automaton of destruction with a clockwork brain, Moustachio was the receptionist at Project Skin Horse. He had a limited long-term memory and needed wax cylinders from cold storage to remember his earlier days. His legs were removed by the British government as a safety precaution. He thought and spoke like a 19th-century gentleman and had little regard for or knowledge of new technology.

A young adult, introduced in the "I Can Fly" story arc,[6] video game obsessive Nick was unknowingly co-opted into "Project Whirligig". The black military program intended to weaponize him by secretly installing him in a virtual reality, then removing his brain and implanting it in a black V-22 Osprey–a military tiltrotor aircraft–and finally subconsciously training Nick to control the Osprey as if it were his own body. In spite of Nick's love of shooting virtual bad guys, he abhored the thought of killing real people, and so joined the Skin Horse team.

Konstantin

Russian black ops agent who joined the American police force after "very accidental and surprising death of Yeltsin." He was introduced in the "Cowardly Lion" story arc,[7] and later became an Annex One security guard.

Dr. Virginia Lee

Introduced in the "I Can Fly" story arc[8] as a mad scientist wannabe and member of a secret organization known only as "Anasigma". She was involved in the creation of both U.N.I.T.Y. and "Project Whirligig". Dr. Lee was specifically hired by Anasigma to reverse engineer Mad Scientist technology into "sane" technology, which she could do with moderate success. Unity was her first successful project, but others included the Brain-O-Mat that produced brain matter to feed zombies, and prevented them from feeding on living humans.

Dr. Lee had an ongoing romantic interest with Nick, but neither party was willing to discuss or admit to this for years — even to themselves.

Captain Bram

A Canadian mad scientist who created Sweetheart and her pack mates, and developed a virus to afflict humans with lycanthropy. When his application to the American Kennel Club to recognize the "Genetically-Engineered Super Battle Dog" as a recognizable breed was denied, he vowed to force the organization to change its position by conquering the United States using said dogs.[9] Bram died before accomplishing his goal, however, and his corpse was reluctantly eaten by his creations.[10]

Chris and Marcie

Two nerdy scientists working for the Department of Irradiation in Annex One, Chris and Marcie were often found gaming with (and within) Nick. Tip and Marcie briefly dated until she dumped him for Chris in the "Tin Soldier" story arc.[11]

Dr. Berenice "Tigerlily" Jones

Blaxploitation-themed mad scientist brought in on a work release program to repair Moustachio during the "Brave Little Toasters" story arc.[12]

Gold Bug

The mysterious benefactor of Project Skin Horse, who was known to Gavotte.