Sex-limited genes

Because sex-limited genes are present in both sexes but only expressed in one, this allows the unexpressed genes to be hidden from selection. On a short-term scale, this means that during one generation, only the sex that expresses the sex-limited trait(s) of interest will be affected by selection. The remaining half of the gene pool for these traits will be unaffected by selection because they are hidden (unexpressed) in the genes of the other sex. Since a portion of the alleles for these sex-limited traits are hidden from selection, this occurrence has been termed 'storage-effect'. On a long-term scale, this storage effect can have significant effects on selection, especially if selection is fluctuating over a long period of time. It is inarguable that selection will fluctuate over time with varying levels of environmental stability. For example, fluctuations in population density can drive selection on sex-limited traits. In less dense populations, females will have less opportunity to choose between males for reproduction. In this case, attractive males may experience both reduced reproductive success and increased predation pressure. Thus, selection on males for sex-limited traits such as increased size (elephant seals) and weaponry (claws on fiddler crabs, horns on rhinoceros beetles) will change direction with fluctuation in population density.[12]

John Parsch and Hans Ellegren defined "genes that differ in expression between females and males" as sex-biased genes. While this definition is more broad, sex-limited genes are certainly included in this category. One of the key principles of sex-biased gene expression that Parsch and Ellegren stressed in their paper in February 2013[13] is that of rapid evolution. They assert that a gene's sex bias can vary among different types of tissues throughout the body or throughout development, making the level of sex bias a fluid, rather than static, property. This makes it possible, then, that the rapid evolution seen in sex-biased genes is not an inherent property of their sex bias, but a property of some other feature. The paper offers expression breadth, the number of tissue types in which the genes are expressed, as an example of a feature correlated to sex-biased genes. It is known that genes with limited expression (in only one type of tissue) generally evolve faster than those with a higher expression breadth, and sex-biased genes are often restricted in their expression, such as to only the testes or ovaries. Thus, it is likely that sex-biased (including sex-limited) genes will evolve faster than the average genetic information. Parsch and Ellegren also assert that "sex-biased genes expressed only in sex-limited reproductive tissues evolve faster than unbiased genes that are expressed only in a single, non-reproductive tissue." That is, genes that have a bias toward any kind of reproductive tissue (testes or ovaries) seem to show faster evolution than genes expressed in non-gonadal tissues, despite the number of tissues in which they are expressed. This makes sense in the context of genes with reproductive function evolving more quickly, a generally observed pattern in evolutionary biology.

Sexual antagonism occurs when two species have conflicting optimal fitness strategies concerning reproduction (see link in introduction paragraph). Multiple matings is a classic example of competing optimal strategies. Males, who typically have a much lower overall investment in reproduction, may benefit from more frequent matings. Females, however, invest much more in reproduction and can be endangered, harmed, or even killed by multiple matings.[14]

Animal behavior (see ethology) encompasses so many disciplines that it is impossible not to see it in some capacity in almost all primary literature involving live animals. While the examples above certainly contain aspects of animal behavior, a more overt example of it in relation to sex-limited traits is detailed in a Teplitsky et al. paper (2010) centering on breeding time in red-billed gulls.[15] This experiment deals with breeding time, an aspect of reproductive biology. Reproduction and sexual behavior are two key aspects of animal behavior, as they are universally expressed in some way throughout the animal kingdom.

Breeding time in red-billed gulls is expressed only in females, because only females lay eggs. Male care, however, affects female breeding performance substantially. This qualifies breeding time as a sex-limited trait because it is expressed only in one sex but can be affected by both (similarly to Hosken's beetle experiment above). By following a natural population of red-billed gulls for 46 years, Teplitsky et al. came to an unexpected conclusion - while laying date (aka breeding time) is only expressed in females, the trait is only heritable in males. This is atypical because sex-limited traits are almost always heritable within the sex in which they are expressed.

For this species, the timing of egg-laying has much to do with male behavior. Males can affect female reproductive success so strongly because for the 20 days up to egg-laying, females spend up to 80% of their time in the nest. This leaves males with the responsibility of providing food regularly and securing (and maintaining) a high-quality territory for nesting. This phenomenon of the genetics of one individual affecting those of another individual is known as indirect genetic effects. For this population, at least, possible explanations for this atypical heritability pattern exist. While controlling female health and safety, males are responsible for the timing of the start of courtship feeding, as well. These populations also typically have excesses of females, allowing males to exert even further choice in the form of mate choice. These factors in combination give males a great opportunity to express their "laying date genotype". In spite of the presence of directional selection and significant male heritability for breeding time, no advancement of breeding time was seen during the 46 years of this experiment. This does not discount the significance of the paper's other results however - one of the most significant being that here a "female trait (laying date) is largely determined by genetic characteristics of its mate".[15]