Phosphomethylpyrimidine synthase
Phosphomethylpyrimidine synthase (EC 4.1.99.17, thiC (gene)) is an enzyme with systematic name 5-amino-1-(5-phospho-D-ribosyl)imidazole formate-lyase (decarboxylating, 4-amino-2-methyl-5-phosphomethylpyrimidine-forming).[1][2][3] This enzyme catalyses the following chemical reaction
- 5-amino-1-(5-phospho-D-ribosyl)imidazole + S-adenosyl-L-methionine 4-amino-2-methyl-5-phosphomethylpyrimidine + 5′-deoxyadenosine + L-methionine + formate + CO
| Phosphomethylpyrimidine synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 Phosphomethylpyrimidine synthase dimer, Arabidopsis thaliana | |||||||||
| Identifiers | |||||||||
| EC no. | 4.1.99.17 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
This enzyme binds a 4Fe-4S cluster.
The starting material is 5-aminoimidazole ribotide, which undergoes a rearrangement reaction via radical intermediates which incorporate the blue, green and red fragments shown into the product.[3][4]
References
- Chatterjee A, Li Y, Zhang Y, Grove TL, Lee M, Krebs C, Booker SJ, Begley TP, Ealick SE (December 2008). "Reconstitution of ThiC in thiamine pyrimidine biosynthesis expands the radical SAM superfamily". Nature Chemical Biology. 4 (12): 758–65. doi:10.1038/nchembio.121. PMC 2587053. PMID 18953358.
- Martinez-Gomez NC, Poyner RR, Mansoorabadi SO, Reed GH, Downs DM (January 2009). "Reaction of AdoMet with ThiC generates a backbone free radical". Biochemistry. 48 (2): 217–9. doi:10.1021/bi802154j. PMC 2654281. PMID 19113839.
- Chatterjee A, Hazra AB, Abdelwahed S, Hilmey DG, Begley TP (November 2010). "A "radical dance" in thiamin biosynthesis: mechanistic analysis of the bacterial hydroxymethylpyrimidine phosphate synthase". Angewandte Chemie. 49 (46): 8653–6. doi:10.1002/anie.201003419. PMC 3147014. PMID 20886485.
- Begley, Tadhg P. (2006). "Cofactor biosynthesis: An organic chemist's treasure trove". Natural Product Reports. 23 (1): 15–18. doi:10.1039/b207131m. PMID 16453030.
External links
- Phosphomethylpyrimidine+synthase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
External links
Media related to Phosphomethylpyrimidine synthase at Wikimedia Commons
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