Lee Byung-heon (biochemist)

• March 1989 – February 1993: Teaching assistant, department of biochemistry, school of medicine, KNU, South Korea
• May 1993 – April 1996: Primary physician, Public Health Center of Changyeong-gun, Gyeongju, South Korea
• March 1996 – February 1998: Instructor, department of biochemistry, school of medicine, Dongguk University, Gyeongju, South Korea
• June 1997 – August 1997: Visiting investigator, MD Anderson Cancer Center, Houston, Texas, United States
• October 1998 – February 2002: Assistant professor, department of biochemistry, school of medicine, Dongguk University, Gyeongju, South Korea
• February 2001 – July 2003: Visiting investigator, The Burnham Institute for Medical Research, San Diego, United States
• August 2003 – present: Professor, department of biochemistry and cell biology, school of medicine, KNU, South Korea; associate dean for research and international programs, school of medicine, KNU, South Korea

• 1989–present: Korean Society of Molecular Biology and Biochemistry
• 1990–present: Korean Society of Molecular Cell Biology
• 2003–present: American Association for Cancer Research
• 2005–present: American Society of Molecular Imaging

Phage display selection of tissue-specific homing peptides and biomarker-sensing peptides: Phage peptide display is a powerful tool for identifying peptides that selectively bind to a target protein or cells (see figure below). Using this technology, our lab has identified diverse homing peptides, including the bladder tumor-targeting peptide, atherosclerotic plaque-homing peptide, and IL-4 receptor-binding peptide. We also have been working on selecting peptides that specifically recognize or sense biomarker proteins of cardiovascular diseases and cancers.

• Acharya, B; Wang, K; Kim, IS; Kang, W; Moon, C; Lee, BH (2013). "In vivo imaging of myocardial cell death using a peptide probe and assessment of long-term heart function". Journal of Controlled Release. 172 (1): 367–73. doi:10.1016/j.jconrel.2013.08.294. PMID 24021357.
• Jeon, J. O.; Kim, S.; Choi, E.; Shin, K.; Cha, K.; So, I. S.; Kim, S. J.; Jun, E.; Kim, D.; Ahn, H. J.; Lee, B. H.; Lee, S. H.; Kim, I. S. (2013). "Designed Nanocage Displaying Ligand-Specific Peptide Bunches for High Affinity and Biological Activity". ACS Nano. 7 (9): 7462–71. doi:10.1021/nn403184u. PMID 23927443.
• Lee, B. H.; Kwon, T. H. (2013). "Application of Phage Display for Ligand Peptidomics to Identify Peptide Ligands Binding to AQP2-Expressing Membrane Fractions". The Low Molecular Weight Proteome. Methods in Molecular Biology. Vol. 1023. pp. 181–9. doi:10.1007/978-1-4614-7209-4_12. ISBN 978-1-4614-7167-7. PMID 23765627.
• Bae, S. M.; Kim, J. H.; Chung, S. W.; Byun, Y.; Kim, S. Y.; Lee, B. H.; Kim, I. S.; Park, R. W. (2013). "An apoptosis-homing peptide-conjugated low molecular weight heparin-taurocholate conjugate with antitumor properties". Biomaterials. 34 (8): 2077–2086. doi:10.1016/j.biomaterials.2012.11.020. PMID 23245333.
• Mentlein, R.; Von Kolszynski, M.; Sprang, R.; Lucius, R. (1990). "Proline-specific proteases in cultivated neuronal and glial cells". Brain Research. 527 (1): 159–162. doi:10.1016/0006-8993(90)91076-S. PMID 2282478.
• Nam, J. -O.; Son, H. -N.; Jun, E.; Cha, K.; Lee, B. -H.; Park, R. -W.; Kim, I. -S. (2012). "FAS1 Domain Protein Inhibits VEGF165-Induced Angiogenesis by Targeting the Interaction between VEGFR-2 and v 3 Integrin". Molecular Cancer Research. 10 (8): 1010–1020. doi:10.1158/1541-7786.MCR-11-0600. PMID 22710795.
• Ha, Y. S.; Lee, H. Y.; An, G. I.; Kim, J.; Kwak, W.; Lee, E. J.; Lee, S. M.; Lee, B. H.; Kim, I. S.; Belay, T.; Lee, W.; Ahn, B. C.; Lee, J.; Yoo, J. (2012). "Synthesis and evaluation of a radioiodinated bladder cancer specific peptide". Bioorganic & Medicinal Chemistry. 20 (14): 4330–5. doi:10.1016/j.bmc.2012.05.049. PMID 22705190.
• Kim, J. H.; Bae, S. M.; Na, M. H.; Shin, H.; Yang, Y. J.; Min, K. H.; Choi, K. Y.; Kim, K.; Park, R. W.; Kwon, I. C.; Lee, B. H.; Hoffman, A. S.; Kim, I. S. (2012). "Facilitated intracellular delivery of peptide-guided nanoparticles in tumor tissues". Journal of Controlled Release. 157 (3): 493–499. doi:10.1016/j.jconrel.2011.09.070. PMID 21945679.
• Lee, G. Y.; Kim, J. H.; Oh, G. T.; Lee, B. H.; Kwon, I. C.; Kim, I. S. (2011). "Molecular targeting of atherosclerotic plaques by a stabilin-2-specific peptide ligand". Journal of Controlled Release. 155 (2): 211–217. doi:10.1016/j.jconrel.2011.07.010. PMID 21781994.
• Wang, K.; Na, M. H.; Hoffman, A. S.; Shim, G.; Han, S. E.; Oh, Y. K.; Kwon, I. C.; Kim, I. S.; Lee, B. H. (2011). "In situ dose amplification by apoptosis-targeted drug delivery". Journal of Controlled Release. 154 (3): 214–217. doi:10.1016/j.jconrel.2011.06.043. PMID 21763738.
• Lee, M. J.; Wang, K.; Kim, I. S.; Lee, B. H.; Han, H. S. (2011). "Molecular Imaging of Cell Death in an Experimental Model of Parkinson's Disease with a Novel Apoptosis-Targeting Peptide". Molecular Imaging and Biology. 14 (2): 147–155. doi:10.1007/s11307-011-0497-z. PMID 21567253.
• He, X.; Na, M. H.; Kim, J. S.; Lee, G. Y.; Park, J. Y.; Hoffman, A. S.; Nam, J. O.; Han, S. E.; Sim, G. Y.; Oh, Y. K.; Kim, I. S.; Lee, B. H. (2011). "A Novel Peptide Probe for Imaging and Targeted Delivery of Liposomal Doxorubicin to Lung Tumor". Molecular Pharmaceutics. 8 (2): 430–438. doi:10.1021/mp100266g. PMID 21222482.
• Mun, G. I.; Kim, I. -S.; Lee, B. -H.; Boo, Y. C. (2010). "Endothelial Argininosuccinate Synthetase 1 Regulates Nitric Oxide Production and Monocyte Adhesion under Static and Laminar Shear Stress Conditions". Journal of Biological Chemistry. 286 (4): 2536–2542. doi:10.1074/jbc.M110.180489. PMC 3024748. PMID 21106532.
• Wang, K.; Purushotham, S.; Lee, J. Y.; Na, M. H.; Park, H.; Oh, S. J.; Park, R. W.; Park, J. Y.; Lee, E.; Cho, B. C.; Song, M. N.; Baek, M. C.; Kwak, W.; Yoo, J.; Hoffman, A. S.; Oh, Y. K.; Kim, I. S.; Lee, B. H. (2010). "In vivo imaging of tumor apoptosis using histone H1-targeting peptide". Journal of Controlled Release. 148 (3): 283–291. doi:10.1016/j.jconrel.2010.09.010. PMID 20869411.
• Lee, E. B.; Jeon, H. S.; Yoo, S. S.; Choi, Y. Y.; Kang, H. G.; Cho, S.; Cha, S. I.; Choi, J. E.; Park, T. I.; Lee, B. H.; Park, R. W.; Kim, I. S.; Kang, Y. M.; Kim, C. H.; Jheon, S.; Jung, T. H.; Park, J. Y. (2010). "Polymorphisms in Apoptosis-Related Genes and Survival of Patients with Early-Stage Non-Small-Cell Lung Cancer". Annals of Surgical Oncology. 17 (10): 2608–2618. doi:10.1245/s10434-010-1082-4. PMID 20422457.
• Jesudasan, M.; John, T. J.; Gupta, B. L.; Bhujwala, R. A.; Shriniwas (1990). "Multiresistant Salmonella typhi in India". Lancet. 336 (8709): 252. doi:10.1016/0140-6736(90)91783-7. PMID 1973803.
• Hong, H. Y.; Lee, H. Y.; Kwak, W.; Yoo, J.; Na, M. H.; So, I. S.; Kwon, T. H.; Park, H. S.; Huh, S.; Oh, G. T.; Kwon, I. C.; Kim, I. S.; Lee, B. H. (2008). "Phage display selection of peptides that home to atherosclerotic plaques: IL-4 receptor as a candidate target in atherosclerosis". Journal of Cellular and Molecular Medicine. 12 (5b): 2003–2014. doi:10.1111/j.1582-4934.2008.00189.x. PMC 4506166. PMID 19012727.
• Hong, H. Y.; Choi, J. S.; Kim, Y. J.; Lee, H. Y.; Kwak, W.; Yoo, J.; Lee, J. T.; Kwon, T. H.; Kim, I. S.; Han, H. S.; Lee, B. H. (2008). "Detection of apoptosis in a rat model of focal cerebral ischemia using a homing peptide selected from in vivo phage display". Journal of Controlled Release. 131 (3): 167–172. doi:10.1016/j.jconrel.2008.07.020. PMID 18692101.
• Thapa, N; Hong, HY; Sangeetha, P; Kim, IS; Yoo, J; Rhee, K; Oh, GT; Kwon, IC; Lee, BH (2008). "Identification of a peptide ligand recognizing dysfunctional endothelial cells for targeting atherosclerosis". Journal of Controlled Release. 131 (1): 27–33. doi:10.1016/j.jconrel.2008.07.013. PMID 18680772.
• Kwon, MK; Nam, JO; Park, RW; Lee, BH; Park, JY; Byun, YR; Kim, SY; Kwon, IC; Kim, IS (2008). "Antitumor effect of a transducible fusogenic peptide releasing multiple proapoptotic peptides by caspase-3". Molecular Cancer Therapeutics. 7 (6): 1514–1522. doi:10.1158/1535-7163.MCT-07-2009. PMID 18566222.
• Lee, Y. -J.; Choi, H. -J.; Lim, J. -S.; Earm, J. -H.; Lee, B. -H.; Kim, I. -S.; Frokiaer, J.; Nielsen, S.; Kwon, T. -H. (2008). "A novel method of ligand peptidomics to identify peptide ligands binding to AQP2-expressing plasma membranes and intracellular vesicles of rat kidney". AJP: Renal Physiology. 295 (1): F300–F309. doi:10.1152/ajprenal.00006.2008. PMID 18480184.
• Nam, J. O.; Jung, M. Y.; Thapa, N.; Lee, B. H.; Park, R. W.; Kim, I. S. (2008). "T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity αvβ3 and α5β1 integrins". Experimental & Molecular Medicine. 40 (2): 196–207. doi:10.3858/emm.2008.40.2.196. PMC 2679300. PMID 18446058.
• Thapa, N.; Kim, S.; So, I. S.; Lee, B. H.; Kwon, I. C.; Choi, K.; Kim, I. S. (2008). "Discovery of a phosphatidylserine-recognizing peptide and its utility in molecular imaging of tumour apoptosis". Journal of Cellular and Molecular Medicine. 12 (5a): 1649–1660. doi:10.1111/j.1582-4934.2008.00305.x. PMC 3918081. PMID 18363834.
• Thapa, N.; Lee, B. H.; Kim, I. S. (2007). "TGFBIp/βig-h3 protein: A versatile matrix molecule induced by TGF-β". The International Journal of Biochemistry & Cell Biology. 39 (12): 2183–2194. doi:10.1016/j.biocel.2007.06.004. PMID 17659994.
• Park, S. -H.; Choi, M. -J.; Song, I. -K.; Choi, S. -Y.; Nam, J. -O.; Kim, C. -D.; Lee, B. -H.; Park, R. -W.; Park, K. M.; Kim, Y. -J.; Kim, I. -S.; Kwon, T. -H.; Kim, Y. -L. (2007). "Erythropoietin Decreases Renal Fibrosis in Mice with Ureteral Obstruction: Role of Inhibiting TGF-beta-Induced Epithelial-to-Mesenchymal Transition". Journal of the American Society of Nephrology. 18 (5): 1497–1507. doi:10.1681/ASN.2005080866. PMID 17389738.
• Lee, S. -M.; Lee, E. -J.; Hong, H. -Y.; Kwon, M. -K.; Kwon, T. -H.; Choi, J. -Y.; Park, R. -W.; Kwon, T. -G.; Yoo, E. -S.; Yoon, G. -S.; Kim, I. -S.; Ruoslahti, E.; Lee, B. -H. (2007). "Targeting Bladder Tumor Cells in vivo and in the Urine with a Peptide Identified by Phage Display". Molecular Cancer Research. 5 (1): 11–19. doi:10.1158/1541-7786.MCR-06-0069. PMID 17259343.
• Lee, B. H.; Bae, J. S.; Park, R. W.; Kim, J. E.; Park, J. Y.; Kim, I. S. (2006). "Βig-h3 triggers signaling pathways mediating adhesion and migration of vascular smooth muscle cells through αvβ5 integrin". Experimental & Molecular Medicine. 38 (2): 153–161. doi:10.1038/emm.2006.19. PMID 16672769.
• Kim, Y. J.; Kim, H. N.; Park, E. K.; Lee, B. H.; Ryoo, H. M.; Kim, S. Y.; Kim, I. S.; Stein, J. L.; Lian, J. B.; Stein, G. S.; Van Wijnen, A. J.; Choi, J. Y. (2006). "The bone-related Zn finger transcription factor Osterix promotes proliferation of mesenchymal cells". Gene. 366 (1): 145–151. doi:10.1016/j.gene.2005.08.021. PMID 16314050.
• Lee, B. H.; Ruoslahti, E. (2005). "Α5β1 integrin stimulates Bcl-2 expression and cell survival through Akt, focal adhesion kinase, and Ca2+/calmodulin-dependent protein kinase IV". Journal of Cellular Biochemistry. 95 (6): 1214–1223. doi:10.1002/jcb.20488. PMID 15962308.
• Nam, J. -O.; Jeong, H. W.; Lee, B. H.; Park, R. W.; Kim, I. S. (2005). "Regulation of Tumor Angiogenesis by Fastatin, the Fourth FAS1 Domain of ig-h3, via v 3 Integrin". Cancer Research. 65 (10): 4153–4161. doi:10.1158/0008-5472.CAN-04-2705. PMID 15899806.
• Lee, S. J.; Jeon, H. S.; Jang, J. S.; Park, S. H.; Lee, G. Y.; Lee, B. H.; Kim, C. H.; Kang, Y. M.; Lee, W. K.; Kam, S.; Park, R. W.; Kim, I. S.; Cho, Y. L.; Jung, T. H.; Park, J. Y. (2004). "DNMT3B polymorphisms and risk of primary lung cancer". Carcinogenesis. 26 (2): 403–409. doi:10.1093/carcin/bgh307. PMID 15528220.
• Park, S. W.; Bae, J. S.; Kim, K. S.; Park, S. H.; Lee, B. H.; Choi, J. Y.; Park, J. Y.; Ha, S. W.; Kim, Y. L.; Kwon, T. H.; Kim, I. S.; Park, R. W. (2004). "Beta ig-h3 promotes renal proximal tubular epithelial cell adhesion, migration and proliferation through the interaction with α3β1 integrin". Experimental & Molecular Medicine. 36 (3): 211–219. doi:10.1038/emm.2004.29. PMID 15272232.
• Lee, S. H.; Bae, J. S.; Park, S. H.; Lee, B. H.; Park, R. W.; Choi, J. Y.; Park, J. Y.; Ha, S. W.; Kim, Y. L.; Kwon, T. H.; Kim, I. S. (2003). "Expression of TGF-beta-induced matrix protein betaig-h3 is up-regulated in the diabetic rat kidney and human proximal tubular epithelial cells treated with high glucose". Kidney International. 64 (3): 1012–1021. doi:10.1046/j.1523-1755.2003.00158.x. PMID 12911551.
• Nam, J. -O.; Kim, J. E.; Jeong, H. W.; Lee, S. J.; Lee, B. H.; Choi, J. Y.; Park, R. W.; Park, J. Y.; Kim, I. S. (2003). "Identification of the alphavbeta3 integrin-interacting motif of betaig-h3 and its anti-angiogenic effect". Journal of Biological Chemistry. 278 (28): 25902–25909. doi:10.1074/jbc.M300358200. PMID 12704192.
• Kim, J. E.; Kim, S. J.; Jeong, H. W.; Lee, B. H.; Choi, J. Y.; Park, R. W.; Park, J. Y.; Kim, I. S. (2003). "RGD peptides released from βig-h3, a TGF-β-induced cell-adhesive molecule, mediate apoptosis". Oncogene. 22 (13): 2045–2053. doi:10.1038/sj.onc.1206269. PMID 12673209.
• Kim, J. -E.; Jeong, H. W.; Nam, J. O.; Lee, B. H.; Choi, J. Y.; Park, R. W.; Park, J. Y.; Kim, I. S. (2002). "Identification of Motifs in the Fasciclin Domains of the Transforming Growth Factor-beta -induced Matrix Protein beta ig-h3 That Interact with the alpha vbeta 5 Integrin". Journal of Biological Chemistry. 277 (48): 46159–46165. doi:10.1074/jbc.M207055200. PMID 12270930.

• Peptides for targeting apoptotic cells and uses thereof US 8217012 KR 20100029232 JP 2009273462 EP 2279201 CN 102177174
• Polypeptide specifically bound to phosphatidylserine and the use thereof US 8133971 KR 20090091589 JP 2011515075 EP 2280024 CN 101965356
• Peptide for diagnosing, preventing and treating atherosclerosis and uses thereof US 7968081 KR 20080090610
• Bladder tumor-targeting peptide and use thereof US 7745410
• Use of a peptide that interacts with alphaV beta3 integrin of endothelial cells EP 1620122
• Novel use of isolated polypeptide comprising four FAS-1 domains, EM1 domain and RGD motif US 2008274955 WO 2005099743
• Target-aiming drug delivery system for diagnosis and treatment of cancer containing liposome labeled with peptides which specifically targets interleukin-4 receptors, and manufacturing method thereof US 2012294931 EP 520281 KR 20110076836
• Peptide which passes through blood-brain barrier and targets apoptosis of neurodegenerative brain disease site and uses thereof US 2012288447 KR 20110053119 WO 2011059263
• Uses of apoptotic cell-targeting peptides, label substances and liposomes containing a therapeutic agent for preventing, treating or therapeutically diagnosing apoptosis-related diseases WO 2011059262
• Stroke-targeting peptide and use thereof US 2011150764 KR 20090125440

• Gowri Rangaswamy Gunassekaran: Post-doctoral fellow (from India)
• Guruprasath Padmanaban : PhD student (from India)
• Jung Hyun-Kyung (정현경): PhD student (from South Korea)
• Chi Lianhua : PhD student (from China)
• Sri Murugan Poongkavithai Vadevod: PhD student (from India)
• Md. Enamul Haque: PhD student (from Bangladesh)
• Fatima Khan: PhD student (from India)
• Smirti Gurung: PhD student (from India)
• Lee Ji-young (이지영): lab manager

• Sangeetha Purushotham: Post-doctoral fellow (from India)
• Bodhraj Acharya: post-doctoral fellow (from Nepal)
• Kai Wang: post-doctoral fellow (from China)
• Jung Jae-Yeop (정재엽): Master's student (from South Korea)
• Jung Joo-Hee (정주희): Master's student (from South Korea)
• Jung Yu-Min (정유민):Master's student (from South Korea)