Heptachlorodibenzo-p-dioxin

1,2,3,4,6,7,8-Heptachlorodibenzo-para-dioxin (often referred to as 1,2,3,4,6,7,8-HpCDD) is a polychlorinated derivative of dibenzo-p-dioxin and can therefore be categorized as polychlorinated dibenzo-p-dioxin (PCDD), a subclass of dioxins which includes 75 congeners. HpCDD is the dibenzo-p-dioxin which is chlorinated at positions 1, 2, 3, 4, 6, 7, and 8. It is a polycyclic heterocyclic organic compound, since HpCDD contains multiple cyclic structures (two benzene rings connected by a 1,4-dioxin ring) in which two different elements (carbon and oxygen) are members of its rings.[1] HpCDD has molecular formula C12HCl7O2 and is an off-white powder, which is insoluble in water.[2]

Heptachlorodibenzo-p-dioxin
Names
Preferred IUPAC name
1,2,3,4,6,7,8-Heptachlorooxanthrene
Other names
  • 1,2,3,4,6,7,8-Heptachlorodibenzo-para-dioxin
  • 1,2,3,4,6,7,8-HpCDD
Identifiers
3D model (JSmol)
ChEBI
ChemSpider
ECHA InfoCard 100.223.051
EC Number
  • 694-835-3
KEGG
UNII
UN number 2811
  • InChI=1S/C12HCl7O2/c13-2-1-3-10(7(17)4(2)14)21-12-9(19)6(16)5(15)8(18)11(12)20-3/h1H
    Key: WCLNVRQZUKYVAI-UHFFFAOYSA-N
  • C1=C2C(=C(C(=C1Cl)Cl)Cl)OC3=C(O2)C(=C(C(=C3Cl)Cl)Cl)Cl
Properties
C12HCl7O2
Molar mass 425.29 g·mol−1
Appearance Off-white powder
1.9 x 10−3 mg/L
Hazards
GHS labelling:
GHS07: Exclamation markGHS08: Health hazardGHS09: Environmental hazard
Danger
H319, H335, H341, H410
P201, P202, P261, P264, P271, P273, P280, P281, P304+P340, P305+P351+P338, P308+P313, P312, P337+P313, P391, P403+P233, P405, P501
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
Infobox references

History

Dioxins are mostly by-products of industrial processes such as the manufacturing of pesticides, bleaching of paper pulp or combustion processes such as waste incineration.[3] This means that heptachlorodibenzo-p-dioxin does not have a common use. In 1998, Professor Sharon Beder argued that large companies have tried to play down the seriousness of the toxic problems of dioxin. She described her concern in her paper ‘The Dioxin Controversy: Spilling over into Schools’.[4] Companies responded by stating that Beder’s paper was based on ‘fear and emotion’ and not on science.[5]

Metabolism

The halflife of heptachlorodibenzo-p-dioxin is calculated to be 3.6 years. This estimation was based on the analysis of fat tissue biopsies collected with an interval of 28 months from on 14-year-old girl who for a period of about 2–3 years had been exposed to technical pentachlorophenol. The half-lives of Hexachlorodibenzo-p-dioxin and octachlorodibenzo-p-dioxin were estimated to be 3.5 and 2 years, respectively.[6]

The effects of chlorodibenzo-p-dioxin exposure was examined in rats over a 13-week period. Hepatic accumulation was associated with alterations of several biochemical parameters. The following alterations were discovered: Ethoxyresorufin-O-deethylase activity was elevated 40-fold over controls; total cytochrome p450 content doubled and exhibited a 2 nanometer blue shift in the Soret maximum for the reduced hemoprotein-CO complex. Cytochrome p450c and cytochrome p450d were significantly increased, while cytochrome p450b levels were unaltered. Nearly all the dioxincongeners present in liver were represented by octachlorodibenzo-p-dioxin, with a slight amount of heptachlorinated-p-dioxin.[7]

Mechanism of action

Dioxins in general are able to bind to the AH receptor. This cell protein initiates effects of most of dioxin-like chemicals. The exact function of the protein of the cell is unknown, but the protein plays a role in rhythmic functions and organ development.

When a dioxin enters the cell and binds to the AH receptor, a complex is formed with another protein, ARNKT. This heterodimer (which is in fact a transcription factor) binds to the DNA. The complex can inhibit or activate unknown genes.[8]

Toxicity

Chloracne, an acne-like eruption of blackheads, cysts, and pustules, is associated with exposure to pentachlorophenol contaminated with heptachlorodibenzo-p-dioxin. This association was first made when individuals employed in the manufacturing of pentachlorophenol were examined. It was discovered that direct contact with the pentachlorophenol lead to significantly increased risk of cloracne.[9] More human studies on the toxicity of heptachlorodibenzo-p-dioxin are not available. However, animal studies showed non-human toxicity levels: LD50 rabbit, oral > 5000 mg/kg, LD50 rat, oral > 5000 mg/kg,[10] and LD50 guinea pig, oral > 600 ug/kg.[11]

Heptachlorodibenzo-p-dioxin is not classifiable as to its carcinogenicity to humans.[12]

The abiothic degradation of heptachlorodibenzo-p-dioxin has been estimated. The rate constant for the vapor phase reaction of heptachlorodibenzo-p-dioxin with photochemically produced hydroxyl radicals has been estimated to be 1.3x10−12 cu cm/molecule-sec at 25 °C which corresponds to an atmospheric half-life of 12.3 days at an atmospheric concentration of 5x105 hydroxyl radicals per cu cm.[13]

Adverse effects

The known adverse effects for dioxin compounds are (as previously mentioned) carcinogenicity. Furthermore, chloroacne, disturbances in tooth development and other developmental effects at high concentration are known adverse effects of HpCDD. There is a very wide range of health effects because the mechanism can have all sorts of implications on the genome (see mechanism of action). HpCDD is known to cause lung cancer and anemia in rats.[14] However, there are not many studies on humans that assess the health risk factors of HpCDD. HpCDD seems to be more potent once it is circulating the body in comparison with other PCDD’s,[15] making it more toxic than other PCDD’s. This probably has to do with the high substitution of chloro groups. Dioxins in general are hard to break down efficiently. This causes bioaccumulation and environmental persistence.[16]

Effects on animals

Immunotoxicity

HpCDD is known to suppress the antibody response of C57B1/6 mice to sheep erythrocytes, a macrophage and T-cell-dependent antigen. In vivo approaches have been applied to characterize the sensitivity of suppression of the antibody response of C57B1/6 mice by an acute oral exposure to HpCDD. Suppression of the antibody response was observed after HpCDD administration at various times prior to or following antigen challenge.[17]

Carcinogenicity

HpCDD is indicated as a potent liver tumor promotor in female (and only female) rats. In primary cultures of hepatocytes of female rats, co-mitogenic actions of HpCDD and congeners are mediated by the aryl-hydrocarbon (AH) receptor. These actions are enhanced by estrogens. HpCDD alone is relatively ineffective in the promotion of tumors in female rats livers, but when DNA-synthesis is stimulated by epidermal growth factor, HpCDD acts as a co-mitogen. These effects are observed for different congeners of polychlorinated dibenzo-p-dioxins, based on their affinity with the aryl-hydrocarbon receptor.[18]

References
  1. IUPAC Nomenclature of Organic Chemistry - http://www.acdlabs.com/iupac/nomenclature/79/r79_13.htm (visited: 14-03-2017), PubChem, compound summary for CID 78968 (1,4-Dioxin) - https://pubchem.ncbi.nlm.nih.gov/compound/1_4-Dioxin#section=Top (visited: 14-03-2017)
  2. Compound summary for CID 37270 - https://pubchem.ncbi.nlm.nih.gov/compound/37270#section=Experimental-Properties (visited: 14-03-2017)
  3. ToxFAQs: Chemical Agent Briefing Sheets (CABS)
  4. ) 'The dioxin controversy: spilling over into schools', Australian Science Teachers' Journal, November 1998, pp. 28-34
  5. Global Spin: The Corporate Assault on Environmentalism, Scribe Publications, p. 154
  6. WHO; Environmental Health Criteria 88: Polychlorinated Dibenzo-para-dioxins and Dibenzofurans p.262 (1989)
  7. Couture LA et al.; Toxicol Appl Pharmacol 93 (1): 31-46 (1988)
  8. Synopsis on dioxins and PCBs - http://www.julkari.fi/handle/10024/80313 (visited: 14-03-2017)
  9. O'Malley MA et al.; Am J Ind Med (17) 4: 411-22 (1990).
  10. USEPA/Office of Pesticide Programs; Reregistration Eligibility Decision Document - Ethalfluralin. p.7-11. EPA 738-R-95-001 (March 1995) Available from, as of May 18th, 2007
  11. WHO; Environmental Health Criteria 88: Polychlorinated Dibenzo-para-dioxins and Dibenzofurans p.141 (1989)
  12. Atkinson R; Environ Toxicol Chem 7: 435-42 (1988) (2) IARC; IARC Monograph on the Evaluation of the Carcinogenic Risk of Chemicals to Humans 15: 47 (1977) (3) Lyman WJ et al.; Handbook of Chem Property Estimation Methods NY: McGraw-Hill p. 7-4 (1982)
  13. IARC. Monographs on the Evaluation of the Carcinogenic Risk of Chemicals to Humans. Geneva: World Health Organization, International Agency for Research on Cancer, 1972-PRESENT
  14. K K Rozman; Delayed acute toxicity of 1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin (HpCDD), after oral administration, Obeys Haber's rule of inhalation toxicology. Toxicol Sci 1999; 49 (1): 102-109. doi:10.1093/toxsci/49.1.102
  15. Van Ede KI, van Duursen MBM, van den Berg M. Evaluation of relative effect potencies (REPs) for dioxin-like compounds to derive systemic or human-specific TEFs to improve human risk assessment.
  16. Claes Bernes: Persistent organic pollutants. Swedish Environmental Protection Agency, Stockholm 1998. ISBN 91-620-1189-8.
  17. Mechanisms of 1,2,3,4,6,7,8-heptachlorodibenzo-p-dioxin (HpCDD)-induced humoral immune suppression: evidence of primary defect in T-cell regulation, Kerkvliet NI, Brauner JA, Toxicol Appl Pharmacol, 1987 Jan;89(1):148
  18. 2,3,7,8-Tetrachlorodibenzo-p-dioxin and ethinylestradiol as co-mitogens in cultured rat hepatocytes, Schrenk D, Karger A, Lipp HP, Bock KW, Carcinogenisis, 1992 Mar;13(3):453-6.
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