Glycan array

Glycan arrays,[1] like that offered by the Consortium for Functional Glycomics (CFG), National Center for Functional Glycomics (NCFG) and Z Biotech, LLC, contain carbohydrate compounds that can be screened with lectins, antibodies or cell receptors to define carbohydrate specificity and identify ligands. Glycan array screening works in much the same way as other microarray that is used for instance to study gene expression DNA microarrays or protein interaction Protein microarrays.

Glycan arrays are composed of various oligosaccharides and/or polysaccharides immobilised on a solid support in a spatially-defined arrangement.[2] This technology provides the means of studying glycan-protein interactions in a high-throughput environment. These natural or synthetic (see carbohydrate synthesis) glycans are then incubated with any glycan-binding protein such as lectins, cell surface receptors or possibly a whole organism such as a virus. Binding is quantified using fluorescence-based detection methods.

Applications

Glycan arrays have been used to characterize previously unknown biochemical interactions. For example, photo-generated glycan arrays have been used to characterize the immunogenic properties of a tetrasaccharide found on the surface of anthrax spores.[3] Hence, glycan array technology can be used to study the specificity of host-pathogen interactions. [4]

Early on, glycan arrays were proven useful in determining the specificity of the Hemagglutinin (influenza) of the Influenza A virus binding to the host and distinguishing across different strains of flu (including avian from mammalian). This was shown with CFG arrays [5] as well as customised arrays.[6] Cross-platform benchmarks led to highlight the effect of glycan presentation and spacing on binding.[7]

Glycan arrays are possibly combined with other techniques such as Surface Plasmon Resonance (SPR) to refine the characterisation of glycan-binding. For example, this combination led to demonstrate the calcium-dependent heparin binding of Annexin A1 that is involved in several biological processes including inflammation, apoptosis and membrane trafficking.[8]

References
  1. Carroll GT, Wang D, Turro NJ, Koberstein JT (2006). "Photochemical Micropatterning of Carbohydrates on a Surface". Langmuir. 22: 2899–2905. doi:10.1021/la0531042.
  2. Oyelaran O, Gildersleeve JC (Oct 2009). "Glycan arrays: recent advances and future challenges". Curr Opin Chem Biol. 13 (4): 406–413. doi:10.1016/j.cbpa.2009.06.021. PMC 2749919. PMID 19625207.
  3. Wang D, Carroll GT, Turro NJ, Koberstein JT, Kováč P, Saksena R, Adamo R, Herzenberg LA, Herzenberg LA, Steinman L (2007). "Photogenerated glycan arrays identify immunogenic sugar moieties of Bacillus anthracis exosporium". Proteomics. 7: 180–184. doi:10.1002/pmic.200600478.
  4. Geissner A, Anish C, Seeberger PH (Feb 2014). "Glycan arrays as tools for infectious disease research". Curr Opin Chem Biol. 18: 38–45. doi:10.1016/j.cbpa.2013.11.013. PMID 24534751.
  5. Stevens J, Blixt O, Tumpey TM, Taubenberger JK, Paulson JC, Wilson IA (Apr 2006). "Structure and receptor specificity of the hemagglutinin from an H5N1 influenza virus". Science. 312 (5772): 404–410. Bibcode:2006Sci...312..404S. doi:10.1126/science.1124513. PMID 16543414.
  6. Childs RA, Palma AS, Wharton S, Matrosovich T, Liu Y, Chai W, Campanero-Rhodes MA, Zhang Y, Eickmann M, Kiso M, Hay A, Matrosovich M, Feizi T (Sep 2009). "Receptor-binding specificity of pandemic influenza A (H1N1) 2009 virus determined by carbohydrate microarray". Nat Biotechnol. 27 (9): 797–799. doi:10.1038/nbt0909-797. PMC 3771066. PMID 19741625.
  7. Wang L, Cummings RD, Smith DF, Huflejt M, Campbell CT, Gildersleeve JC, Gerlach JQ, Kilcoyne M, Joshi L, Serna S, Reichardt NC, Parera Pera N, Pieters RJ, Eng W, Mahal LK (Jun 2014). "Cross-platform comparison of glycan microarray formats". Glycobiology. 24 (6): 507–17. doi:10.1093/glycob/cwu019. PMC 4001710. PMID 24658466.
  8. Horlacher T, Noti C, de Paz JL, Bindschädler P, Hecht ML, Smith DF, Fukuda MN, Seeberger PH (Apr 2011). "Characterization of annexin A1 glycan binding reveals binding to highly sulfated glycans with preference for highly sulfated heparan sulfate and heparin". Biochemistry. 50 (13): 2650–9. doi:10.1021/bi101121a. PMC 3068229. PMID 21370880.
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