Chondroitin ABC lyase
Chondroitin ABC lyase (EC 4.2.2.20 EC 4.2.2.21, chondroitinase, chondroitin ABC eliminase, chondroitinase ABC) is an enzyme with systematic name chondroitin ABC lyase.[1][2] This enzyme catalyses the following chemical reaction
- Eliminative degradation of polysaccharides containing 1,4-beta-D-hexosaminyl and 1,3-beta-D-glucuronosyl or 1,3-alpha-L-iduronosyl linkages to disaccharides containing 4-deoxy-beta-D-gluc-4-enuronosyl groups
| chondroitin-sulfate-ABC endolyase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 4.2.2.20 | ||||||||
| CAS no. | 9024-13-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
| chondroitin-sulfate-ABC exolyase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 4.2.2.21 | ||||||||
| CAS no. | 9024-13-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
This enzyme acts on chondroitin 4-sulfate, chondroitin 6-sulfate and dermatan sulfate.
Uses
Following a spinal cord injury, this enzyme can be used to erode scar tissue that can interfere with regeneration.[3]
References
- Yamagata T, Saito H, Habuchi O, Suzuki S (April 1968). "Purification and properties of bacterial chondroitinases and chondrosulfatases". The Journal of Biological Chemistry. 243 (7): 1523–35. doi:10.1016/S0021-9258(18)93574-X. PMID 5647268.
- Saito H, Yamagata T, Suzuki S (April 1968). "Enzymatic methods for the determination of small quantities of isomeric chondroitin sulfates". The Journal of Biological Chemistry. 243 (7): 1536–42. doi:10.1016/S0021-9258(18)93575-1. PMID 4231029.
- Bartus K, James ND, Didangelos A, Bosch KD, Verhaagen J, Yáñez-Muñoz RJ, Rogers JH, Schneider BL, Muir EM, Bradbury EJ (April 2014). "Large-scale chondroitin sulfate proteoglycan digestion with chondroitinase gene therapy leads to reduced pathology and modulates macrophage phenotype following spinal cord contusion injury". The Journal of Neuroscience. 34 (14): 4822–36. doi:10.1523/JNEUROSCI.4369-13.2014. PMC 3972714. PMID 24695702.
- "Gene therapy improves limb function following spinal cord injury". EurekAlert! (Press release). 1 April 2014.
External links
- Chondroitin+ABC+lyase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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