Aminoglycoside N6'-acetyltransferase
In enzymology, an aminoglycoside N6'-acetyltransferase (EC 2.3.1.82) is an enzyme that catalyzes the chemical reaction
- acetyl-CoA + kanamycin-B CoA + N6'-acetylkanamycin-B
| aminoglycoside N6'-acetyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.3.1.82 | ||||||||
| CAS no. | 56467-65-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
Thus, the two substrates of this enzyme are acetyl-CoA and kanamycin B, whereas its two products are CoA and N6'-acetylkanamycin-B.
This enzyme belongs to the family of transferases, specifically those acyltransferases transferring groups other than aminoacyl groups. The systematic name of this enzyme class is acetyl-CoA:kanamycin-B N6'-acetyltransferase. Other names in common use include aminoglycoside 6'-N-acetyltransferase, aminoglycoside-6'-acetyltransferase, aminoglycoside-6-N-acetyltransferase, and kanamycin acetyltransferase.
Structural studies
As of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 1S3Z, 1S5K, 1S60, and 2A4N.
References
- Le Goffic F, Martel A (1974). "[Resistance to aminosides induced by an isoenzyme, kanamycin acetyltransferase]". Biochimie. 56 (6–7): 893–7. doi:10.1016/s0300-9084(74)80512-2. PMID 4614862.
- Benveniste R, Davies J (1971). "Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor". Biochemistry. 10 (10): 1787–96. doi:10.1021/bi00786a009. PMID 4935296.
- Dowding JE (1977). "Mechanisms of gentamicin resistance in Staphylococcus aureus". Antimicrob. Agents Chemother. 11 (1): 47–50. doi:10.1128/aac.11.1.47. PMC 351917. PMID 836013.