Alpha-N-acetylneuraminate alpha-2,8-sialyltransferase
In enzymology, an alpha-N-acetylneuraminate alpha-2,8-sialyltransferase (EC 2.4.99.8) is an enzyme that catalyzes the chemical reaction
- CMP-N-acetylneuraminate + alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-R CMP + alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D- galactosyl-R
| alpha-N-acetylneuraminate alpha-2,8-sialyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.99.8 | ||||||||
| CAS no. | 67339-00-8 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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Thus, the two substrates of this enzyme are CMP-N-acetylneuraminate and alpha-N-acetylneuraminyl-2,3-beta-D-galactosyl-R, whereas its 3 products are CMP, alpha-N-acetylneuraminyl-2,8-alpha-N-acetylneuraminyl-2,3-beta-D-, and galactosyl-R. This enzyme participates in 4 metabolic pathways: glycosphingolipid biosynthesis - neo-lactoseries, glycosphingolipid biosynthesis - globoseries, glycosphingolipid biosynthesis - ganglioseries, and glycan structures - biosynthesis 2.
This enzyme belongs to the family of transferases, specifically those glycosyltransferases that do not transfer hexosyl or pentosyl groups. The systematic name of this enzyme class is CMP-N-acetylneuraminate:alpha-N-acetylneuraminyl-2,3-beta-D-galactos ide alpha-2,8-N-acetylneuraminyltransferase. Other names in common use include cytidine monophosphoacetylneuraminate-ganglioside GM3, alpha-2,8-sialyltransferase, ganglioside GD3 synthase, ganglioside GD3 synthetase sialyltransferase, CMP-NeuAc:LM1(alpha2-8) sialyltransferase, GD3 synthase, and SAT-2.
References
- Eppler CM, Morre DJ, Keenan TW (1980). "Ganglioside biosynthesis in rat liver: alteration of sialyltransferase activities by nucleotides". Biochim. Biophys. Acta. 619 (2): 332–43. doi:10.1016/0005-2760(80)90081-8. PMID 7407217.
- Higashi H, Basu M, Basu S (1985). "Biosynthesis in vitro of disialosylneolactotetraosylceramide by a solubilized sialyltransferase from embryonic chicken brain". J. Biol. Chem. 260 (2): 824–8. PMID 3838172.
- McCoy RD, Vimr ER, Troy FA (1985). "CMP-NeuNAc:poly-alpha-2,8-sialosyl sialyltransferase and the biosynthesis of polysialosyl units in neural cell adhesion molecules". J. Biol. Chem. 260 (23): 12695–9. PMID 4044605.
- Yohe HC, Yu RK (1980). "In vitro biosynthesis of an isomer of brain trisialoganglioside, GT1a". J. Biol. Chem. 255 (2): 608–13. PMID 6766128.