5-oxoprolinase (ATP-hydrolysing)
In enzymology, a 5-oxoprolinase (ATP-hydrolysing) (EC 3.5.2.9) is an enzyme that catalyzes the chemical reaction
- ATP + 5-oxo-L-proline + 2 H2O ADP + phosphate + L-glutamate
| 5-oxoprolinase (ATP-hydrolyzing) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.5.2.9 | ||||||||
| CAS no. | 9075-46-1 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
The 3 substrates of this enzyme are ATP, 5-oxo-L-proline, and H2O, whereas its 3 products are ADP, phosphate, and L-glutamate.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in cyclic amides. The systematic name of this enzyme class is 5-oxo-L-proline amidohydrolase (ATP-hydrolysing). Other names in common use include pyroglutamase (ATP-hydrolysing), oxoprolinase, pyroglutamase, 5-oxoprolinase, pyroglutamate hydrolase, pyroglutamic hydrolase, L-pyroglutamate hydrolase, 5-oxo-L-prolinase, and pyroglutamase. This enzyme participates in glutathione metabolism.
References
- Van der Werf P, Orlowski M, Meister A (1971). "Enzymatic conversion of 5-oxo-L-proline (L-pyrrolidone carboxylate) to L-glutamate coupled with cleavage of adenosine triphosphate to adenosine diphosphate, a reaction in the -glutamyl cycle". Proc. Natl. Acad. Sci. U.S.A. 68 (12): 2982–5. doi:10.1073/pnas.68.12.2982. PMC 389574. PMID 5289242.